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Full Length
Research Paper
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In vitro
cleaning of sugarcane (Saccharum officinarum)
explants against the viral infections by application of the
viral inhibitors (DHHT and virazol): A preliminary study
Asseng Charles Carnot1*, Ambang Zachée2,
Pomozkov Youri Ivanovich3 and Keuldeuch Marina
Alexandrovna3
1University
of Douala, Department of Biology of the Vegetal Organism P.
O. Box 24157, Cameroon.
2University
of Yaounde I, Department of Plant Biology and Physiology P.
O. Box 812, Yaounde, Cameroon.
3Russian
University of the People Friendship, 117198 Moscow,
Miklouxo-Maklaya street, N°8/1, Russia Federation.
*Corresponding author. E-mail:
carnotass@yahoo.fr.
Tel.:
(237) 99 981197.
Accepted
6 March, 2009 |
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Abstract |
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The
objective of this work was to cleanse explants of Saccharum
officinarum cultivated in vitro using the viral
inhibitors DHHT and virazol. The basic vegetal material
constituted seeds of 4
varieties of sugarcane, Х99-055, Х97-029, X99-071 and X97-035,
obtained from the CIRAD of Guadeloupe.
The explants of foliar, stem and root origin size 0.7 - 1.5
mm were cultivated on modified medium of Mourassigue and Skougou.
The viral inhibitors introduced in the culture medium to
proportions of 1%. The diagnosis of the viruses were made by
symtomatologic tests confirmed by enzyme linked immunosorbent
assay (ELISA). 5 viruses (SCMV, CMV, RLMV and YDBV) were
screened. The use of the virazol and DHHT makes possible to
obtain 40, 55, 35 and 60 % explants respectively free from SCMV,
CMV, RLMV and YDBV on the X97-029 variety for explants size 1.2
- 1.5 mm. The results show that it is possible to obtain a
seed-bearer material of sugarcane free from some viruses by the
method of cleaning using the viral inhibitors on explants of
larger cuts (1.2 - 1.5 mm).
Key words:
Saccharum officinarum, explants, viral inhibitors |
