Effects of two different soil amendments on the biocontrol
biological control agents (BCA)
against Ralstonia wilt on ginger
Hongxia Liu1, Yunpeng Wang1, Yuming
Luo2, Hetong Yang3 and Jianhua Guo1*
of Plant Pathology, College of Plant Protection, Nanjing
Agricultural University, Engineering Center of Bioresource
Pesticide in Jiangsu Province, Key Laboratory of Monitoring
and Management of Crop Diseases and Pest Insects, Ministry
of Agriculture, Nanjing 210095, China.
Key Laboratory for Eco-Agricultural Biotechnology around
Hongze Lake, School of Life Science, Huaiyin Normal
University, Huaian 223300, China.
Center of Shandong Academy of Sciences, No. 19, Keyuan Road,
Jinan 250014, China.
*Corresponding author. E-mail:
Accepted 30 April, 2012
In this research, we developed an integrated approach to
control Ralstonia wilt on ginger. Two organic composts
(maize powder and soybean residue) were combined with one
combination (the aqueous solution of Bacillus subtilis
and Bacillus cereus) and two single (B. subtilis
1JN2 and Bacillus megaterium B1301) biological
control agents (BCA) which together showed significant
biocontrol efficacies in our previous study. Biocontrol
efficacy of each treatment was measured. According to the
results, a 3 - 30% biocontrol efficacy improvement was
obtained by addition of the two organic composts compared to
the BCA treatment. The best biocontrol activity (73.7%) was
obtained from the combination of B.
B1301 and maize powder. Colonization ability and the impacts
on the bacterial community structure of each BCA were
determined by denaturing gradient gel electrophoresis (DGGE).
The DGGE fingerprints gave us an insight into the
rhizosphere of the plant. And there is no noticeable
bacterial structure shift, which means the exotic BCAs by
artificial inoculation have little impacts on the local
organism community. Additionally, the colonization ability
of the tested BCAs was excellent and they could be detected
at 120 days post inoculation. Furthermore, the available N,
P, K and organic matter of rhizosphere soil from each
treatment was measured. The organic matter did not show
significant change among each treatment, while maize powder,
soybean residue and BCA 1JN2 enhanced the available N, P and
K significantly ranging from 79 - 133%, 166 - 251% and 252 -
368%, respectively compared to the blank control.
Integrated management, biological control agents (BCA),
organic compost, ginger wilt.