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An efficient and rapid method for protein detection with an
example of sulfide-quinone reductase expressed in
Escherichia coli
Yan-fen He#, Ai-ling Zhang# and Jia-qi
Li*
Guangdong Provincial Key Lab of Agro-animal Genomics and
Molecular Breeding, College of Animal Science, South China
Agricultural University, Guangzhou, Guangdong 510642,
China.
*Corresponding author: E-mail:
jqili@scau.edu.cn.
Tel: +86-020-85283519. Fax: +86-020-85280740.
#These authors contributed equally to this paper
Accepted 30 March, 2012 |
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Conventional Coomassie brilliant blue (CBB) staining is
widely used in protein detection as it is inexpensive and
reproducible. Conversely, it is also very
time-consuming and cumbersome. In this study,
CBB(R-250)-microwaved-water (CMW) was used to detect the
sulfide-quinone reductase (SQR) protein expressed in
Esherichia coli by staining and destaining in under 30
min. The CMW method has similar detection capabilities as
the conventional CBB method. However, compared with the CBB
protocol, it did not produce pungent odors, and was more
efficient and rapid, which saved more than 4 to 10 h. This
method is an acceptable alternative for the preliminary
detection of protein expression.
Key words:
CMW method, Coomassie brilliant blue (R-250),
sulfide-quinone reductase, microwave irradiation, tap water. |
