Molecular characterization and novel genetic variability in
leptin (obese) gene of mithun (Bos frontalis)
P. P. Dubey1,3#*, D. S. Gour1#,
Prashant Singh1, Anubhav Jain1, Amit
Kumar2, S. C. Gupta1 and Dinesh Kumar1
Bureau of Animal Genetic Resources, Karnal, Haryana-132 001,
of Animal Genetics, Indian Veterinary Research Institute,
Izatnagar, Bareilly-243 122, India.
of Animal Genetics and Breeding, GADVASU, Ludhiana-141004
*Corresponding author. E-mail:
#Authors contributed equally
Accepted 12 April, 2012
The present study was undertaken with the objectives of
sequencing, characterization and single nucleotide
polymorphisms (SNPs) identification of mithun leptin gene.
The mithun leptin gene (3420 bp) was sequenced, compared
with other species and phylogenetic tree were constructed.
Single-strand conformation polymorphism (SSCP) showed three
patterns in both exon-2 and exon-3 of the gene. Nucleotide
sequences from all patterns revealed three novel SNPs. In
exon-2 at 25th codon, one SNP (AGC/TGC) with
amino acid changed from serine to cysteine, and at 37th
codon one silent mutation (ACA/ACG) was detected. In exon-3
at 72nd codon, one silent mutation (CCG/CCA)
was also identified. This preliminary study of existing gene
variants can be used for further association studies,
thereby establishing conservation of mithun germplasm.
Leptin gene, mithun, phylogeny, single nucleotide