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Hepatoxicity of aqueous
extract and fractionated methanol extract of Phytolacca
americana by isolated rat liver perfusion system
Mohammad Karami1*, Farshad Naghshvar2,
Sodabah Saeidnia3 and Nada Omrani1
1Pharmaceutical
Sciences Research Center, School of Pharmacy, Medical
Sciences University of Mazandaran, Sari, Iran.
2Pathology
Department, School of Medical. Medical Sciences University
of Mazandaran, Sari, Iran.
3Pharmacognos
Department, Medical plants Research Center. Medical Sciences
University of Tehran, Iran.
*Corresponding author. E-mail:
toxkarami@yahoo.com.
Tel: +98 151 3543081-3. Fax: +98 151-3543084.
Accepted
14 October, 2009 |
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Phytolacca americana is a perennial plant native to North America and other parts
of the world. It is well known for several medicinal
properties despite being considered to have digestive
toxicity (especially hepatotoxicity). Our objective is to
examine whether extract of aerial parts of P. americana
could produce biochemical changes by Isolated Rat Liver
Perfusion (IRLP) system, which is ideal for studying
biochemical alterations of chemicals with minimum
neural-hormonal effects. In this study, the liver was
perfused with Kerbs-Henseleit buffer, containing different
concentration of aqueous extract of the aerial parts of
P. americana (10, 20, 40, 50,100 mg/kg) and CHCl3,
ethanol and methanol fraction (10, 20, 40 mg/kg) were added
to the buffer and perfused for 2 h. During the perfusion,
many factors including amino-transferase activities were
assessed as indicator of liver viability. Consequently,
sections of liver tissue were examined for any histo-pathological
changes. The results showed that histo-pathological changes
in liver tissues were related in a dose-dependent manner to
methanol extract of aerial parts Phytolacca americana
concentrations. Doses of 50, 100 mg/kg caused significant (P
< 0.05) histo-pathological changes.
Key
words:
Phytolacca americana,
Liver perfusion, Kerbs-Henseleit buffer, histo-pathological
changes, amino-transferees activity.
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