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Validated high performance liquid chromatographic (HPLC)
method for analysis of zerumbone in plasma
Eltayeb Elamin M. Eid1, Ahmad Bustamam Abdul1*,
Adel S. Al-Zubairi1,4, Mohamed Aspollah Sukari2
and Rasedee Abdullah3
1Laboratory
of Cancer Research MAKNA-UPM, Institute of Bioscience,
University Putra Malaysia, 43400 Serdang, Selangor DE,
Malaysia.
2Department
of Chemistry, Faculty of Science, University
Putra Malaysia, 43400 Serdang, Selangor DE, Malaysia.
3Department
of Veterinary Pathology and Microbiology, Faculty of
Veterinary Medicine,
University Putra Malaysia,
43400, Serdang, Selangor DE, Malaysia.
4Department
of Biochemistry and Molecular Biology, Faculty of Medicine
and Health Sciences, University of Sana’a, Sana’a, Yemen.
*Corresponding author. E-mail:
ahmadbstmm@yahoo.com
or
adelalzubairi@hotmail.com.
Tel: +603-89462124 Fax: +603-89462101.
Accepted 11 November, 2009 |
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Zerumbone (ZER) is a
sesquiterpene derived from Zingiber zerumbet smith, family
Zingiberaceae. It has been shown to possess anti-cancer and
apoptosis-inducing properties against various human tumour
cells as well as in vivo against a number of induced
malignancies in mice. In this study a
simple, specific and accurate high performance liquid
chromatographic method for determination of ZER in
micro-volumes human plasma (|
1.5 ml) was developed and validated. ZER and its analogue α-Humulene
as internal standard were easy recovered by simple one step
plasma protein precipitation using acetonitrile and
separated in isocratic mobile phase, on reverse phase-C18
column. The effluent was monitored by Photodiode Array (PDA)
detector and at a flow rate of 1.0 ml/min. The linearity of
proposed method was 2 – 15 µg/ml. The intra-day and
inter-day coefficient of variation and percent error values
of the method were less than 15% and mean recovery was more
than 90% for both ZER and α-Humulene. This method was found
to be precise, specific, accurate and robust for detection
and analysis of ZER in human plasma.
Key words:
ZER, humulene, HPLC, human plasma. |
