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Nitrogen supplements
effect on amylase production by Aspergillus niger
using cassava whey medium
C. E. Oshoma1*, E. E. Imarhiagbe2,
M. J. Ikenebomeh1 and H. E. Eigbaredon1
1Department
of Microbiology, University of Benin, P.M.B. 1154, Benin
City, Nigeria.
2Green
Consultants Ltd, Edo State Environmental Laboratory, House
Annex, Sapele Road, Benin City, Nigeria.
*Corresponding author. E-mail:
oshomacy@yahoo.com.
Tel: +234 8033732608.
Accepted
27 April, 2009 |
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The production of amylase by Aspergillus niger on
three cassava whey media in liquid shake culture was
compared. The supplemented cassava whey (SCW) medium
exhibited gave amylase activity of 495 U/ml. Biomass cropped
was 1.63 g/l in the SCW medium. Yeast extract employed as a
nitrogen supplement increased biomass yield of A. niger
to 2.75 g/l with maximum amylase activity of 643 U/ml.
Sodium nitrate (NaNO3) as nitrogen supplement had
the lowest biomass yield of 0.77 g/l and amylase activity of
206 U/ml. Thus yeast extract as nitrogen supplement of
cassava whey medium supported maximum production of amylase
and biomass of A. niger.
Key
words:
Amylase, cassava whey, Aspergillus niger, yeast
extract. |
