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Phenotypic
and genotypic characterization of lactic acid bacteria
isolated from Azerbaijani traditional dairy products
Amarela
Terzic-Vidojevic1*, Maja Tolinacki1,
Milica Nikolic1, Jelena Lozo1, Jelena
Begovic1, Sahib Gurban oglu Gulahmadov2,
Akif Alekperovich Kuliev2, Michèle Dalgalarrondo3,
Jean-Marc Chobert3, Thomas Haertlé3
and Ljubisa Topisirovic1
1Institute
of Molecular Genetics and Genetic Engineering, University of
Belgrade, Vojvode Stepe 444a, P. O. Box 23, 11010 Belgrade,
Serbia.
2Department
of Biochemistry and Biotechnology, Baku State University,
23, Khalilov Street, 370602 Baku, Azerbaijan.
3UR
1268 Biopolymers Interactions Assemblies, National Institute
of Agronomic Research, Functions and Interactions of Dairy
Proteins, B. P. 71627, 44316 Nantes Cedex 3, France.
*Corresponding author. E-mail:
lab6@eunet.rs. Tel.: +381
11 3975960. Fax: +381 11 3975808.
Accepted 2 April, 2009 |
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Studied lactic acid bacteria (LAB) were isolated from two
types of final ready-to-eat artisanal dairy products
(cheeses – Agdas, Sheki and yogurts - Karabakh, Ganja and
Baku) manufactured in Azerbaijan. The Agdas cheese belongs
to the group of semi hard cheeses whilst the Sheki cheese
belongs to hard cheeses. Both of cheeses were produced from
cow’s milk without the addition of the starter cultures. The
Karabakh and Baku yogurts were produced from bovine’s milk
and the Ganja yogurt from buffalo’s milk. Overall 378
isolates were collected from these dairy products and 296 of
them were Gram-positive and catalase-negative. It was
determined using biochemical tests and molecular methods
that four species of LAB were mostly present in these
cheeses:
Lactobacillus plantarum,
Lactobacillus brevis, Lactobacillus paraplantarum
and
Enterococcus faecium while in yogurts, Lactobacillus
delbrueckii subsp.
lactis,
Lactobacillus delbrueckii subsp.
bulgaricus, Streptococcus thermophilus and
Enterococcus faecium dominated. Five
enterococci were producers of antimicrobial compounds.
Key words:
Antimicrobial activity, Azerbaijan, dairy products, DGGE,
non-starter lactic acid bacteria (NSLAB), proteolytic
activity, rep-PCR, 16S rDNA. |
