Full Length Research Paper

An effective disinfection protocol for plant regeneration from shoot tip cultures of strawberry

Chien-Ying Ko^1*, A. M. Al-Abdulkarim², S. M. Al-Jowid², and A. Al-Baiz²

¹The International Cooperation and Development Fund (Taiwan ICDF), Taipei 11157, Taiwan.

²National Agriculture and Animal Resources Research Center, Ministry of Agriculture, Kingdom of Saudi Arabia.

*Corresponding author. E-mail: a0955579973@yahoo.com.tw. Tel.: +966-01-4576780 ext.2322. Fax: +966-01-4584979.

Accepted 3 April, 2009

An effective method of disinfection protocol and micropropagation with an enhanced survival rate of explants and reduced phenol induced browning in strawberry was developed. The survival rate of three genotypes was between 89.2 - 100%. Shoot tip were able to develop into plantlet on a hormone-free MS medium when cultured under dim light (500 lux). Two media, M1 and M2 were chosen to compare the effect on shoot multiplication of three genotypes. The shoot number per responding explants was between 5 - 8 and 11 - 14 after induction.

Key words: Strawberry, micropropagation, shoot tip.