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Effect of
plant growth regulators on callus induction and plant
regeneration in tuber segment culture of potato (Solanum
tuberosum L.) cultivar Diamant
Khadiga G.
Abd Elaleem1, Rasheid S. Modawi1 and
Mutasim M. Khalafalla2*
1Faculty
of Sciences and Technology, Al-Neelain University, Khartoum,
Sudan.
2Commission
for Biotechnology and Genetic Engineering, National Center
for Research P. O. Box 2404, Khartoum, Sudan.
*Corresponding author. E-mail:
mkhalafalla@ncr.sd
or
mutasimhh@hotmail.com.
Accepted 30 March, 2009 |
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The present study was conducted to investigate the effects
of different concentrations and combinations of growth
regulators on callus induction and plant regeneration
of potato (Solanum tuberosum L.) cultivar Diamant.
The tuber segments
were used as explants and
cultured on
Murashige and Skoog (MS)
medium supplemented with different concentrations of
α-naphthalene acetic acid (NAA), 2,4-Dichlorophenoxy acetic
acid (2,4-D), benzyl adenine
(BA) and thidiazeron (TDZ)
alone and 2,4-D in combinations with BA for callus
induction. The best degree for callus formation (6.0) was
obtained on MS medium supplemented with 2,4-D alone at 3.0
mg/l or 2,4-D in combination with BA both at 2.0 mg/l. MS
media supplemented with different levels of BA and TDZ were
employed for shoot regeneration. MS medium containing 5.0
mg/l TDZ was the best for days to shoot initiation, the
highest percentage of callus with shoot (81%) and highest
number of shoot per callus (3.4).
Callus derived shoots were rooted most effectively in
half-strength MS medium containing 0.5 mg/l IBA. The success
of plant tissue culture for in vitro culture of
potato was encouraged by acclimatization of the plantlets in
the greenhouse conditions. Regenerated plants were
morphologically uniform with normal leaf shape and growth
pattern.
Key words:
Potato, 2,4-D,
callus, regeneration, acclimatization. |
