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Genetic variation of
calsarcin-1 gene and association with carcass traits in 3
Chinese indigenous cattle
Dapeng Yang1,
Linsen Zan1,2*, Hongbao Wang1,2,
Yun Ma1, Wanqiang Tian1
and Yingying Zhang1
1College
of Animal Science and Technology, Northwest A and F
University, Yangling, Shaanxi 712100, P. R. China.
2National
Beef Cattle Improvement Center, Yangling, Shaanxi 712100, P.
R. China.
*Corresponding author. E-mail:
zanls@yahoo.com.cn. Tel.: +86 29 87091923. Fax: +86 29
87092164.
Accepted 27
May, 2009 |
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This study aimed to investigate polymorphisms of Calsarcin-1
gene and evaluate its effect on carcass traits in 429
samples of 3 Chinese indigenous cattle breeds, namely, Luxi
(LX), Nanyang (NY) and Jiaxianred (JXR) breeds, PCR products
with a 320 bp fragment of the Calsarcin-1 gene
spanning over a part of intron 2, complete exon 3 and a part
of intron 4 were amplified and sequenced. A synonymous
alteration (NW_001495138:g.16718C>A)
in the exon 3 region of the calsarcin-1 gene was detected
and PCR-SSCP method was then developed to genotype all of
the individuals. The results showed that the allele
frequencies of A/C in LX, NY and IXR breeds were
0.1116/0.8884, 0.1591/0.8409 and 0.0947/0.9053,
respectively. Least squares analysis revealed a significant
effect (P<0.05) of genotypes on backfat thickness and
tenderness
of the animals,
with the AC and AA genotype being favourable compared to the
CC genotype.
Key words:
Calsarcin-1 gene, carcass traits, cattle, SNP. |
