|
Purification and
characterization of the chitosanase from
Aeromonas sp. HG08
Yuying Sun1, Jiquan Zhang2*
and Shujun Wang1
1College
of Marine Science, Huaihai Institute of Technology,
Lianyungang 222005, P. R China.
2Institute
of Oceanology, Chinese Academy of Sciences, Qingdao, 266071,
P. R. China.
*Corresponding author. E-mail:
zhangjiquan@ms.qdio.ac.cn.
Accepted 15
April, 2009 |
|
In this work, the characterization of a chitosanase-producing
bacterium isolated from soil was reported and this strain
was grouped under the genus Aeromonas by virtue of
its morphological, physiological properties and 16S
rDNA gene sequences. It is the first report that the genus
Aeromonas could produce chitosanase. Aeromonas
sp. HG08 could secrete the chitosanase (named AsChi)
with molecular weight of 70 kDa. The optimum pH and
temperature of AsChi was 6.0 and 55°C,
respectively. The activity of AsChi was markedly enhanced by
Mn2+ and inhibited by
Fe3+,
Cu2+ , Ag+ and Hg2+;
additionally, the
activity of AsChi was increased with the degree of
deacetylation (DDA) of chitosan. Through viscosimetric
assay, AsChi probably hydrolyzed chitosan in an endo-type
fashion.
Key
words:
Aeromonas
sp.,
chitosanase,
purification, characterization. |
