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Genetic diversity in
rhizobial isolates determined by RAPDs
K. Rajasundari *, K. Ilamurugu and Logeshwaran, P.
Department
of Agricultural Microbiology, Tamil Nadu Agricultural
University, Coimbatore 641003, India.
*Corresponding author. E-mail:
rajasundarikandaiah@yahoo.in.
Accepted
15 April, 2009 |
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Nine soil rhizobia isolated from different field locations
were subjected to RAPD analysis to study the diversity. It
was found that Rhizobium isolates from Agricultural
Research Station - Aliyar Nagar (ALN 7), Department of
Agricultural Microbiology (SOB 1), isolates from TNAU
Eastern block field No. 36 (EB 36) and Millet Breeding
station field: 9 (MBS 19) were clustered together to form
cluster 1 followed by Rhizobium isolates Millet
Breeding station field: 8 (MBS 9), isolates from the
Department of Agrl. Microbiology, TNAU 14 and COG 15 belongs
to cluster 2 and isolates of Cotton Breeding station field
No: 9 (CBS 9) and Aliyar Nagar (ALN 2) formed the fourth
cluster. Department of Agricultural Microbiology isolate,
COG 15 with TNAU 14 and SOB 1 with Rhizobium ALN 7
were clustered together with more than 50 per cent
similarity. Isolate from Cotton Breeding station field No:
12 (CBS 12) was found to have more divergence (>90 per cent)
to make the separate cluster. The results indicated that
RAPD is a very discriminative and efficient method for
differentiating and studying genetic diversity of
Rhizobium strains.
Key
words:
Rhizobium, RAPD, genetic diversity. |
