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DSN1 deletion is
deleterious to the Saccharomyces cerevisiae while
Dsn1p disrupts nuclear segregation process of Chinese
Hamster Ovary cell
Beow Chin Yiap1, A .K. Radhakrishnan2
and Nadimpalli Ravi S. Varma3*.
1School
of Pharmacy and Health Sciences, Faculty of Medicine and
Health, International Medical University, No. 126, Jalan
19/155B, Bukit Jalil, 57000 Kuala Lumpur, Malaysia.
2Department
of Pathology, Faculty of Medicine and Health, International
Medical University, No. 126, Jalan 19/155B, Bukit Jalil,
57000 Kuala Lumpur, Malaysia.
3Department
of Biological and Agriculture Engineering, University of
California, Davis, 95616, California, USA.
*Corresponding author. E-mail:
rnadimpalli@ucdavis.edu. Tel: 001-530-754-0332. Fax: 530
752-2640.
Accepted
20 June, 2008 |
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Dsn1p is a component of the mind complex (Mtw1p Including
Nnf1p-Nsl1p-Dsn1p) that is essential for the segregation of
chromosome in yeast cells. This protein assists the joining
of kinetochore subunits contacting DNA to those contacting
microtubules in yeast cells. Null mutants of this protein
are not viable while the over-expression of the Dsn1 protein
in yeast cells results in nuclear anomalies and growth
defects. In this paper, we
show that tetrad analysis indicates haplo-insufficiency and
segregational errors in yeast diploid single deletants.
Expression of Dsn1p in CHO has been achieved using the pcDNA
3.1/HIS A expression vector. Analysis by DNA sequencing
showed no changes in the DSN1 DNA sequence. We also showed
that nuclear fragmentation and cellular morphological
changes takes place in CHO cell line that were successfully
transfected with the pcDNA 3.1/HIS A expression vector
containing the Dsn1p.
Key
words:
Saccharomyces cerevisiae, kinetochore, Dsn1p, Chinese
Hamster Ovary (CHO). |
