Full Length Research Paper

Yr10 gene polymorphism in bread wheat varieties

A. Temel¹*, F. Şentürk-Akfırat², F. Ertuğrul³, A. Yumurtacı³, Y. Aydın⁴, T. Talas-Oğraş³, N. Gözükırmızı^1*, N. Bolat⁵, Ö. Yorgancılar⁵, S. Belen⁵, M. Yıldırım⁵, M. Çakmak⁵, E. Özdemir⁵, L. Çetin⁶, Z. Mert⁶, H. Sipahi⁶, S. Albustan⁶, K. Akan⁶, F. Düşünceli⁶ and A. Altınkut Uncuoğlu³

¹Istanbul University, Molecular Biology and Genetics Department, 34118, Vezneciler, Istanbul/Turkey.

²Gebze Institute of Technology, Institute of Higher Technologies, Biology Department, Muallimköy Campus, Gebze-Kocaeli/Turkey.

³TUBITAK, MRC, Institute for Genetic Engineering and Biotechnology, P.O. Box 21 41470, Gebze-Kocaeli/Turkey.

⁴Marmara University, Biology Department, Göztepe 34722, Kadıköy, Istanbul/Turkey.

⁵Anatolian Agricultural Research Institute PK 17, 26010, Eskişehir/Turkey.

⁶Field Crops Research Institute, P.K. 226, 06042 Ulus, Ankara/Turkey.

*Corresponding author. E-mail: asl_temel@yahoo.com. Tel: 0090 212 455 57 00/15110.

Accepted 20 June, 2008

Yellow rust resistance locus Yr10 located on chromosome 1B in Moro and originated from the Turkish line PI178383 was investigated in terms of polymorphism in seven winter type bread wheat cvs. (Triticum aestivum ssp. Aestivum) Altay2000, İzgi2001, Sönmez2001 (yellow rust resistant), Aytın98, ES14, Harmankaya99 (yellow rust susceptible) and PI178383 as control. Exon 1 (1 - 833 bp) and Exon 2 (1989 - 3630 bp) parts of Yr10 were amplified with three primers. Amplification was not observed with E2A primers in Harmankaya99, İzgi2001 and Sönmez2001 cvs, while amplification products were observable at all tested varieties with the other primers. PCR results showed that E2A reverse primer is not able to anneal to the three varieties mentioned above. Sequence analysis and bioinformatics analysis proved that there has been single nucleotide changes especially in the second exon. The most similar sequences to the first exon of Harmankaya99, İzgi01 and Sönmez2001 are AF509535 (Aegilops tauschii NBS-LRR-like gene), AF509534 (A. tauschii NBS-LRR-like gene sequence) and AF509534, respectively. These results could be helpful in revealing divergence between resistant and susceptible varieties.

Key words: Triticum aestivum L., yellow rust, resistance gene, PCR, sequence analysis.