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In vitro
culture of Telfairia
occidentalis under different cytokinins and auxin
combination
Sanusi I. S.*, Odofin W. T., Aladele S. E., Olayode M.
N., Gamra E. O. and Fajimi O.
National
Center for Genetic Resources and Biotechnology, Ibadan, P.
M. B. 5382, Moor Plantation, Ibadan, Nigeria.
*Corresponding author. E-mail:
ibidun2002za@yahoo.co.uk
Abbreviations:
NAA, Naphthalene acetic acid; BAP, benzylaminopurine; IBA,
indolebutyric acid; and IAA, indole-3-acetic acid.
Accepted
16 October, 2007 |
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Telfairia occidentalis
is a tropical vine and has been a good source of iron rich
vegetable to man. It is normally propagated through seeds
but the seeds are recalcitrant in nature. The vegetative
propagation of T. occidentalis has been difficult
hence there is a need to develop an in vitro method.
Nodal cuttings of T. occidentalis was cultured on
Murashige and Skoog (MS; 1962) basal media supplemented with
IBA, IAA, NAA, BAP, and kinetin at different concentrations.
Among all the growth hormones used, IBA and BAP combination
gave the best result for both rooting and shooting while BAP
in combination with NAA showed the highest number of nodes.
The result showed that in vitro growth of T.
occidentalis is hormone specific.
Key
words:
Telfairia occidentalis,
hormones, IBA, IAA, NAA, BAP, kinetin in vitro. |
