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In vitro
plant regeneration from Narbon Vetch (Vicia narbonensis
L.) using cotyledonary node explants
Hayrettin Kendir, Nurdan Sahin-Demirbag, Khalid Mahmood
Khawar*, and Muhammad Aasim
Department
of Field Crops, Faculty of Agriculture, Ankara University
Ankara, 06110, Diskapi, Turkey.
*Corresponding author. E-mail:
kmkhawar@gmail.
Abbreviations: BAP, 6 Benzylaminopurine; NAA, α
Naphthalene acetic acid; IBA, Indole 3 butyric acid; TDZ,
Thidiazuron.
Accepted
18 June, 2008 |
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Narbon vetch (Vicia narbonensis L.) is an
agriculturally important forage plant that widely grows in
an area extending from Central Europe to various parts of
Asia. The study reports axillary shoot regeneration from
cotyledonary node explants obtained from, in vitro
raised seeds of 4 - 5 and 14 - 15 days old seedlings on MS
medium containing 2 - 6 mg/l kinetin-0.1 mg/l
indole-3-butyric acid (IBA).
No shoot regeneration was recorded on 14 - 15 days old
cotyledon node explants. Whereas, 4 - 5 days old cotyledon
node explants showed high regeneration potential with the
highest number of 3.85 shoots per explant, with mean shoot
length of 2.11 cm and shoot regeneration frequency of
93.33%. The shoots obtained from all regeneration media
could be easily rooted after pulse treatment with 50 mg/l
IBA for 7 min.
All of
which were morphologically normal and fertile and easily
established under greenhouse conditions.
Key
words:
Narbon vetch, Vicia narbonensis L, axillary shoot
regeneration, rooting. |
