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HPLC
analysis of water-soluble vitamins (B1, B2, B3, B5, B6) in
in vitro and ex vitro germinated chickpea (Cicer
arietinum L.)
Junaid Aslam2, Mohammed S. Mohajir1,2,
Saeed A. Khan1,2* and Abdul Q. Khan2
1Department
of Pharmaceutical and Medicinal Chemistry,
Al Muhasinah P.O. Box 19099 United Arab Emirates.
2Plant
Tissue Culture Research,
Dubai Pharmacy College, Al Muhasinah P.O. Box 19099 United
Arab Emirates.
*Corresponding author. E- mail:
dr.khan@dpc.edu.
Abbreviations: HPLC, High performance liquid
chromatography; RSD, standard relative deviation; DMRT,
Duncan multiple range test.
Accepted 10 June, 2008 |
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In the present communication, a comparative HPLC analysis of
water-soluble vitamins (B-group) was carried out in in
vitro and ex vitro germinated chickpea (Cicer
arietinum L.) seeds. Quantitative analysis of ex
vitro and in vitro germinated seedlings showed
significant differences in vitamin contents. Maximum amount
with a linear increase in B1
(thiamine HCl),
B2 (riboflavin),
and B3 (nicotinamide)
contents
was noticed up to 9 days old ex vitro grown
seedlings. However, B5 (pantothenic acid)
and B6 (pyridoxine
HCl)
were higher in in vitro germinated seedlings. Thus,
vitamin production was age and culture conditions dependent,
which is discussed in detail. The study revealed that the
germinated chickpea grains can be used for human consumption
with value addition of vitamin B-group in the diet of
vegetarians.
Key words:
Cicer arietinum
L., water-soluble vitamins, in vitro and in vitro
culture. |
