Length Research Paper
essential and non essential amino acids in the single cell
protein (scp) of white rot fungi from wheat straw
J. Khanifar1*, H.
Ghoorchian2, A. R. Ahmadi3 and R.
of Biochemistry, Payame Noor University, Tehran, Iran.
of Biochemistry and Biophysics, University of Tehran,
of Biomedical Science, Alzahra University, Tehran, Iran.
*Corresponding author. E-mail:
Accepted 13 July, 2011
As the constraints related to environmental issues are
becoming quite severe and stringent, it is necessary to
develop optimized systems for food waste treatment. White
rot fungi are ideal parameters for lignocellulosic wastes to
produce microbial protein. In this study wheat straw was
treated with NaOH 2% and heated at 100°C and was inoculated
with Pleurotus florida by solid state fermentation (SSF).
The crude protein was 62.8% per 100 g of dried single cell
protein (SCP). The extracted protein hydrolyzed with HCl 6
Normal, and the amino acids analyzed by A-200 amino nova
analyzer. The profile concentration of non essential amino
acids was: Aspartic acid = 5.22, Serine = 3.6, Glutamic acid
= 6.38, Prolin = 3.2, Glycine = 4.21, Alanine = 6.23,
Cycteine = 1.18, Tyrosine = 2.61 g/100 g of extracted
protein. The concentration of essential amino acids however
has been reported. The comparison of both essential and non
essential amino acids in conclusion, indicates that the
ratio of essential amino acids to total amino acids was
65.6% which clearly proves the effectiveness of this fungus
for enhancing the feed value of agro-residues. All
experiments were carried out in triplicate and result
Pleurotus, microbial protein, lignocellulosic waste,
animal feed, solid state fermentation.