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Effect of
Astragalus membranaceus polysaccharides on oxidative
damage in skeletal muscle of exhaustive exercise rats
Zhi-hong Deng1* and
Qing-lan Hu2
1Kunming University,
Kunming, Yunnan Province, 650214, People’s Republic of
China.
2Kunming University of
Science and Technology, Kunming, Yunnan Province, 650224,
People’s Republic of China.
*Corresponding author. E-mail:
zh_deng95@yahoo.cn .
Accepted 11 July, 2011 |
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The aim of this study was to determine the effects of
Astragalus membranaceus polysaccharides (AMP) on
oxidative damage in skeletal muscle of exhaustive exercise
rats. The animals were divided into four groups at random,
with eight rats in each group. Group 1, assigned as a
control group, received normal saline (vehicle); groups 2 to
4 received AMP at different dosages (50, 100 and 200 mg/kg
bw) respectively for 30 successive days. Rats were made to
perform an exhaustive running test on a treadmill at a final
speed of 30 m/min, 10% grade, at approximately 70 to 75% VO2max.
The mean endurance times of running on a treadmill to
exhaustion of each rat were examined. And skeletal muscle
samples were collected for determining the activity of
superoxide dismutase (SOD), glutathione peroxidase (GSH-Px),
catalase (CAT) and levels of malondialdehyde (MDA). The
results showed groups 2 to 4 significantly increased in
exhaustive exercise time compared with groups 1. MDA
concentration of skeletal muscle of the groups 2 to 4 were
significantly decreased compared with that of the groups 1.
The antioxidant enzyme activities of SOD, GSH-Px and CAT of
skeletal muscle of the groups 2 to 4 significantly increased
compared with that of the groups 1. It is concluded that AMP
supplementation increased performance of exhaustive exercise
of rats. And it is beneficial for increasing antioxidant
status and inhibits oxidative stress induced by exhaustive
exercise.
Key words:
Astragalus membranaceus
polysaccharides, oxidative damage, exhaustive exercise, rats |
