Full Length Research Paper

In vitro embryo rescue culture of F1 progenies from crosses between tetraploid grape and Vitis amurensis Rupr.

Yinshan Guo^#, Yuhui Zhao^#, Kun Li, Zhendong Liu, Hong Lin, Xiuwu Guo* and Chengxiang Li

College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.

*Corresponding author. E-mail: guoxw1959@163.com.  Tel: 86-24-88487147. Fax: 86-24-88487146.

Accepted 5 September, 2011

 Abstract

Using three tetraploid table grape varieties as the female parents and Vitis amurensis Rupr. as the male parent, the effects of inoculation time and medium state on embryo rescue were studied. The results showed that: inoculation time had a great effect on ovule germination percentage. 60 to 70 days after pollination was the best inoculation time, which resulted in the highest ovule germination percentage. Among solid medium, liquid medium, semi-solid medium and liquid over solid double layer medium, the last medium had the highest germination percentage, and then the solid medium. Liquid and semi-solid media were not good.

Key words: Grapes, embryo rescue, inoculation time, medium.