Molecular analysis of genetic diversity in olive cultivars
Azmat Ali Awan1*,
Muhammad Zubair1, Aqib Iqbal2, Sayed
Jaffar Abbas3 and Naushad Ali4
Department, Khyber Pakhtunkhwa, Agriculture University,
of Biotechnology and Genetic Engineering, Khyber Pakhtunkhwa,
Agriculture University, Peshawar, Pakistan.
Center, Agriculture Research Institute, Tarnab, Peshawar,
Breeding Department, Khyber Pakhtunkhwa, Agriculture
University, Peshawar, Pakistan.
*Corresponding author. E-mail:
Accepted 9 August, 2011
The present study was conducted to study the genetic
diversity in the available cultivars of olive flora in
Pakistan. On an average, 15.5 alleles were amplified using
Randomly Amplified Polymorphic DNA (RAPD) molecular primers.
Mean genetic distance estimates ranged from 0.158 to 0.512 (G.D
= 15.8 to 51.2%). Size of scorable fragments ranged from
approximately 250 to >2000 bp. A high level of genetic
dissimilarity (GD= up to 51%) was recorded among Ferugenia
genotype only. Entries were grouped in clusters using
cluster analysis. On the basis of dendrogram, most diverse
genotypes Ferugenia and Coratina were identified, that can
be used in future olive propagation program.
Olive, randomly amplified polymorphic DNA (RAPD) molecular
primers, genetic diversity, Pakistan.