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Afr. J. Agric. Res.


Vol. 6 No. 28



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African Journal of Agricultural Research Vol. 6(28), pp. 6021-6026, 26 November, 2011

Available online at http://www.academicjournals.org/AJAR

DOI: 10.5897/AJAR11.938

ISSN 1991-637X ©2011 Academic Journals

 

 

Full Length Research Paper

 

In vitro pollen germination in stone fruit tree of Rosaceae family

 

Yavar Sharafi

 

Department of Horticulture, Faculty of Agriculture, Shahed University- Opposite Holy Shrine of Imam Khomeini- Khalij Fars Expressway- Tehran- Iran. E-mail: y.sharafi@shahed.ac.ir. Tel: +982151210 and  +989144200882 P. O. Box 18155/159, Postal/ ZIP 3319118651.

 

Accepted 18 August, 2011

 

 Abstract

 

In the tone fruit trees including almond, apricot, plum, prune, sour cherry and sweet cherry, pollen performance includes pollen quantity produced in a flower and pollen quality consists of viability, longevity, morphological homogeneity, pollen germination and pollen tube growth rate which are very important component of fertilization and fruit setting. However, study of pollen traits is one of the most important approaches for growers and breeders. In this research, main pollen traits including germination and tube growth were investigated in some selected cultivars of Prunus species including P. dulcis, P. armeniaca, P. domestica, P. ceracus, P. salicina and P. avium. Pollen traits of 5 cultivars from each species were studied using the in vitro medium containing 15% sucrose, 1% agar 5 ppm H3BO3 (boric acid).  Pollens were planted in the in vitro medium inside the Petri-dishes and incubated at the constant temperature of 24°C for 24 h and then, pollen germination and growth were protected by adding chloroform. Data analysis showed that in all of the studied traits significant differences were observed among six species and cultivars of each species.

 

Key words: Almond, apricot, plum, prune, sour cherry, sweet cherry, Rosaceae, pollen viability, in vitro.

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