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Genetic
diversity in hulless barley based on agromorphological
traits and RAPD markers and comparison them with storage
protein analysis
R. Eshghi* and E. Akhundova
Faculty of Biology, Department Genetics and Darwinism, Baku
State, University, Azerbaijan,
*Corresponding author. E-mail:
rohameshghi@yahoo.com
.
Tel: +99450-7164789.
Accepted 19 November, 2009 |
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Genetic diversity among 63 hulless
barley accessions originating from ICARDA (International
Centre for Agricultural Research in the Dry Area) was
investigated using agromorphological traits and random
amplified polymorphic DNA (RAPD) variation. Among 20
agromorphological traits under study, a considerable
diversity was observed for grain yield per plot, 1000-grain
weight and peduncle length. High estimates of heritability
in broad sense were recorded for plant height, number of
grains per spike and number of tillers. These traits can be
used for indirect improvement of yield. Cluster and
Principal component analysis based on agromorphological
traits could effectively classify the samples. The RAPD-based
genetic similarity ranged from 0.221 to 0.81, with the mean
of 0.481. Cluster analysis based on Jaccard Similarity
Coefficient divided genotypes into 8 different groups. The
average of genetic diversity index for RAPDs and storage
proteins were compared and showed that mean of genetic
diversity index was lesser for RAPDs than storage proteins.
Thus, when resources are a limiting factor and considering
the cost of consumables and work time, seed storage proteins
must be the technique of choice for a first estimation of
genetic variation in hulless barley genetic resource
collections. In RAPD analysis, a special band was observed
which could be used in identifying hulless barley varieties
with low or high
b-glucan.
Key words:
Genetic diversity, hulless barley, agromorphological traits,
RAPDs, storage proteins. |
