|
Genetic diversity in
doubled haploids wheat based on morphological traits,
gliadin protein patterns and RAPD markers
J. Ojaghi* and E. Akhundova
Department
of Genetic and Darwinism, Faculty of Biology, Baku State
University, Azerbaijan.
*Corresponding author.
E-mail:
Javid_804@yahoo.com
.
Tel: +99450-3455674.
Accepted 22
June, 2010 |
|
Genetic
diversity among 102 doubled haploid wheat accessions
originating from CIMMYT were investigated using
morphological traits, gliadin patterns and random amplified
polymorphic DNA (RAPD) variation. Among the morphological
traits under study, the highest amount of diversity was
related to grain yield per plant, number of total tillers
and number of fertile tillers. Principle components analysis
and cluster analysis for morphological traits were used to
effectively classify the samples. Based on these analyses,
three genotypes with maximum yield and the related traits
were determined. In the analysis of gliadins, 48 bands and
47 different patterns were detected. The average genetic
diversity index for these proteins was calculated as H =
0.75. The mean of genetic diversity index was more for RAPDs
than gliadins (H = 0.83). Although during statistical
reviews one pattern in the
w
area was found to have
relations with the trait of spikelet per spike, no
relationship was found between morphological, storage
proteins and RAPDs data. As a result, it seems that applying
only one of these methods is not sufficient to estimate the
genetic diversity. In order to have a clearer picture of the
status of genetic diversity in different populations of
bread wheat, it is recommended that all the three methods be
applied simultaneously.
Key
words:
Genetic diversity, doubled haploids wheat, morphological
traits, gliadin patterns, RAPD marker. |
