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Directional transfer of the
genic multiple allele inherited male sterile line in Chinese
cabbage
Li Cheng-yu, Feng Hui*, Wei Peng, Wang Li-li, Zhang Jun,
Yang Hao-ning, Jiang Nan
Department of Horticulture, Shenyang Agricultural
University, Shengyang 110161
Liaoning, China.
*Corresponding author. Email:
fenghuiaaa@263.net.
Tel.: 86-13898899863. Fax: 86-024-88487143.
Accepted
27 April, 2009 |
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A
“directional transfer program” was designed and carried out
according to the genotype on the male sterility locus of the
target line, in order to improve the utilization of the
multiple-allele inherited male sterile gene in Chinese
cabbage and solve the problems in the transfer process of
this gene. In the program, recurrent backcrossing was
employed to transfer botanical traits, while the genotype
was identified through test cross. In addition, we began to
develop the male sterile line after the transfer of
botanical traits was finished. Following the procedure, the
male sterile gene was successfully transferred from “3A”, a
white leafstalk inbred line in Chinese cabbage, to “206”,
the target green leafstalk inbred line in cylindrical
ecotype. The “GMS104”, a male sterile line with similar
botanical traits to “206” with 100% male sterility and 100%
male sterile plants, was bred. With “GMS104” as the female
parent, an excellent hybridized combination “GMS104×B4” was
finally obtained and it passed the variety certification and
registration of minor crops in Liaoning, China. Based on the
results, it was certified that male sterility and the other
botanical and economic traits could be transferred to a
target line simultaneously following the “directional
transfer program” designed in our study.
Key
words:
Chinese
cabbage, genetic male sterile line, transfer. |
