|
Progeny from the crosses of two antisense potato plants
exhibit ectopic xylem differentiation
Olawole O. Obembe1,3* and Jean-Paul
Vincken1,2
1Laboratory of Plant Breeding, Wageningen University, Box 386 6700AJ
Wageningen, The Netherlands.
2Laboratory of Food Chemistry, Wageningen University Bomenweg 2 /
8129,6700 EV Wageningen, The Netherlands.
3Department of Biological Sciences, Covenant University, P. M. B 1023 Ota,
Ogun State, Nigeria.
*Corresponding author. E-mail:
obembe@covenantuniversity.com. Tel: +2348060164341.
Accepted
20 June, 2008. |
|
Progeny
from the crosses of two transgenic potato lines csr2-1 and
csr4-8, containing two different antisense constructs, csr2
and csr4 had been previously characterized to exhibit
altered tuber production. Histochemical staining and
microscopic examinations of the tubers were made to
investigate cellular phenotype in the tubers. We observed
ectopic proliferation of xylem, which is most pronounced in
the csr2 tubers. Light microscopy of csr2 tubers revealed
that the proliferation of xylem was associated with
lignification of their cell walls. This paper provides
evidence of cellular phenotype as a consequence of the
presence of the antisense construct.
Key
words:
Genetic crossing, microscopy, potato, ectopic xylem, lignin. |
