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Extracellular enzymes from brown-rot fungus Laetiporus
sulphureus isolated from mangrove forests of coastal
Tanzania
Godliving Mtui* and Rose Masalu
Department of Molecular Biology and Biotechnology,
University of Dares Salaam
P. O. Box 35179, Dar es Salaam, Tanzania.
*Corresponding author. E-mail: gmtui@udsm.ac.tz. Or
gmtui@hotmail.com.
Accepted
28 March, 2008 |
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A
brown-rot fungus, Laetiporus sulphureus (Fr.) Murr.,
was isolated from Mbweni, Oyster Bay and Mtoni Mangroves
Forests in Dar es Salaam, Tanzania, and the biochemical
properties of its extracellular enzymes were investigated.
The crude culture filtrate was concentrated by
ultrafiltration. Protein content and lignocellololytic
enzyme activities were measured by photometric methods. The
crude enzyme extract was purified by gel chromatography and
characterized by sodium docecyl sulphate-polyacrylamide gel
electrophoresis (SDS-PAGE). The fungal filtrate had maximum
manganese peroxidase (MnP) of 2.5 U/mL and lignin peroxidase
(LiP) of 1 U/mL, but showed no laccase (Lac) activity. The
enzyme extracts were able to oxidize rhemazol brilliant
blue-R (RBB-R) dye and phenol, and could remove up to 90%
color from raw textile effluent in immobilized culture. The
purified peroxidases showed that the MnP from S.
sulphureus has a molecular weight of 48 kDA. The study
elucidated the extracellular enzymes profile of facultative
marine L. sulphureus and provided basic information
on their potential for biological wastewater treatment
systems.
Key
words: Brown-rot fungi, extracellular enzymes,
biodegradation, electrophoresis. |
