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Enzyme cocktail for enhancing poultry utilisation of cocoa
pod husk
Francis Alemawor1*, Victoria P. Dzogbefia1,
Emmanuel O.K. Oddoye2 and James H. Oldham1
1Department
of Biochemistry and Biotechnology, Kwame Nkrumah University
of Science and Technology, Kumasi, Ghana.
2Cocoa
Research Institute of Ghana, P. O. Box 8, New Tafo–Akim,
Ghana.
*Corresponding author. E-mail:
alefracid2000@yahoo.com.
Tel.: +233-20-8780625. Fax: +233-51-60137
Accepted 23 March, 2009. |
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Cocoa Pod Husk (CPH) is a major by-product from the cocoa
industry in Ghana. Using CPH as a low-cost unconventional
feedstuff is hampered by its poor nutrient composition due
to high level of non-starch polysaccharides including
cellulose, pectin and hemicellulose, which are indigestible
in monogastric livestock such as poultry. An in vitro
enzyme treatment study was conducted to test the effect of
various combinations of selected exogenous fibrolytic
enzymes on the digestibility of CPH feedstuff.
Concentrations of 0.8, 0.6 and 0.8% w/w respectively for
Pentopan®MonoBG, Viscozyme®L and
Pectinex®5XL were observed as appropriate levels
for supplementing CPH feedstuff. Among the enzyme
combinations tested, the Pentopan®MonoBG +
Viscozyme®L, Viscozyme®L + Pectinex®5XL
and Pentopan®MonoBG + Viscozyme®L +
Pectinex®5XL formulae were most effective in
maximising sugar release from CPH feedstuff by 42 - 53%
increase with a corresponding reduction (7 - 14%) in crude
fibre and non-starch polysaccharide fractions (P < 0.05).
The present results suggest that supplementation with
multi-enzymes or blends of exogenous NSP-degrading enzymes
may enhance the capacity of poultry to efficiently digest
and utilise dietary CPH.
Key
words:
Agro-industrial by-product, cocoa pod husk, non-starch
polysaccharides, in vitro treatment, fibrolytic
enzymes, saccharification. |
