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Full Length Research Paper
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Characterization of industrial waste and identification of
potential micro-organism degrading tributyl phosphate
Trupti D. Chaudhari3, Susan Eapen2 and
M. H. Fulekar1*
1Department of Life Sciences, University of Mumbai, Santacruz (E),
Mumbai-400 098, India.
2Nuclear Agriculture Biotechnology Division, Bhabha Atomic Research
centre, Trombay-400 085 Mumbai, India.
3Research Scholar, Environmental Biotechnology Laboratory, Department of
Life Sciences, University of Mumbai, Santacruz (E),
Mumbai-400 098, India.
*Corresponding author. E-mail:
mhfulekar@yahoo.com.
Accepted
6 May, 2009 |
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Abstract |
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The present
research study has been carried out in the waste disposal
site for characterization of physical, chemical and
biological parameters to assess the microbial consortium
present in the contaminated site and to isolate the
potential microorganism for biodegradation of Tributyl
Phosphate. The ambient conditions present in the
contaminated site shows the values: pH (6.61), Temperature
(35.6), Moisture (50.72%), Nutrients; Nitrogen (0.41%),
Phosphorus (27.87 mg/l), and Sulphur (993.5 mg/l)
respectively. The biological parameters studied indicate
Dissolved Oxygen (4.58 mg/l), Biological Oxygen Demand
(4.62 mg/l), Chemical Oxygen Demand (146.1 mg/l). The microbial
consortium identified was found to survive and multiply in
the present environment conditions. Microbial consortium was
sequenced and compared using BLAST, ClustalW and PHYLIP. In
order to identify potential microorganism, microbial
consortium was exposed to increasing concentration of
Tributyl Phosphate viz. 10, 25, 50, 75, 100 mg/l in MSM, the
potential microorganism was found to survive at higher
concentration and utilized it as a sole source of carbon.
This organism was identified as Pseudomonas
pseudoalcaligenes strain DSM 50018T using 16S rRNA
sequencing. This organism was found to have high potential
for degradation of Tributyl Phosphate present in Low Level
Nuclear Waste.
Key
words:
Tributyl phosphate (TBP), low level nuclear waste, 16S rRNA
sequencing, industrial effluent, biodegradation. |
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