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Cogon grass [Imperata cylindrica L. Beauv. var. major] is the only one
species of Imperata, and one of the medicinal plants in Taiwan. The
rhizome can be used for medicinal purposes. In the field alcohol
dehydrogenase (ADH) activity, proline and sodium content in tissues of
Imperata showed variation between wetland (Chuwei population) and the
other two representative non-wetland ecotypes (Neihu and Sarlun
populations). Chuwei ecotype is known tolerant to flood and salt shown
in a flood and salt treatment experiment in previous study. A mass
spectrometry (MS)-based proteomic analysis identified a fast moving
2-phospho-D-glycerate hydrolase (enolase) isoform only in the wetland
ecotype on a native-polyacrylamide gel electrophoresis, whereas a slow
moving enolase isoform was identified only in the non-wetland ecotypes.
In addition, a mitochondrial malate dehydrogenase and a chloroplast
ferredoxin-NADP(H) oxidoreductase were identified only in the wetland
ecotype. These proteomic results provide additional evidence of the
ecotypic variation among Imperata cylindrica ecotypes. The potential use
of this novel approach to identify protein markers is discussed in the
study.
Key words: Imperata
cylindrica,
ecotype, mass spectrometry, proteomic, enolase, malate dehydrogenase,
ferredoxin-NADP(H) oxidoreductase. |
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