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Journal of Medicinal Plants
Research Vol. 5(3), pp.
371–378,
4 February, 2011
ISSN 1996-0875 ©2011 Academic
Journals |
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Full Length Research Paper |
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Detection of bioactive compounds from Spilanthes acmella
(L.) plants and its various in vitro culture products
Tan Chee Leng1, Ning Shu Ping1, Boey Peng
Lim2 and Chan Lai Keng1*
1School
of Biological Sciences, Universiti Sains Malaysia, 11800 Penang,
Malaysia.
2School
of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang,
Malaysia.
*Corresponding author. E-mail:
lkchan@usm.my,
merrilynchan@gmail.com.
Tel: 604-6533828. Fax: 604-6565125.
Accepted 19 October, 2010 |
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Abstract |
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In this study, we analyzed the active chemical compounds of
in vitro plantlets of Spilanthes acmella. With 19
samples, GC-MS (Gas Chromatography-Mass Spectrometry) analysis
revealed that the naturally occurring insecticide,
N-isobutyl-2E, 6Z, 8E-decatrienamide (spilanthol) was present in
mother plant, flower heads and in vitro plantlets of
S. acmella with similar retention time (43.18 to 43.21 min).
N-isobutyl-2E, 4Z, 8Z, 10E- dodecatetraenamide (an isomer of
N-isobutyl-2E, 4E, 8E, 10E- dodecatetraenamide, a potent
mosquito larvicide) was only detected in the in vitro
plantlets of S. acmella. Antioxidant, butylated
hydroxytoluene (BHT) and fatty acids (n-Hexadecanoic acid and
tetradecanoic acid) could be obtained from all the sample
extracts of mother plant, flower heads, in vitro
plantlets, callus, air-dried cells, freeze-dried cells and fresh
cells.
Key words:
Chemical analysis, spilanthol, micropropagation, Spilanthes
acmella. |
