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Journal of Medicinal Plants
Research Vol. 5(3), pp.
456–461,
4 February, 2011
ISSN 1996-0875 ©2011 Academic
Journals |
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Full Length Research Paper |
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Compensatory effects
of curcumin on cisplatin-induced toxicity in rabbit testis
F. M. Kandemir1*,
F. Benzer2, N. C. Yildirim3 and N. Ozdemir1
1Department
of Biochemistry, Faculty of Veterinary Medicine, Firat
University, Elazig, Turkey.
2Veterinary
Control and Research Institute, Elazig, Turkey.
3Department
of Environmental Engineering, Faculty of Engineering, Tunceli
University, Tunceli, Turkey.
*Corresponding author. E-mail:
fmk_03@mynet.com. Tel: +90
4242370000.
Fax: +90 4242338720.
Accepted 20 December, 2010 |
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Abstract |
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Curcumin, a widely used spice and
colouring agent in food, has been shown to possess potent
antioxidant, antitumor promoting and anti-inflammatory
properties in vitro and in vivo. The present study
was designed to investigate the protective effects of curcumin
on changes in the levels of lipid peroxidation and endogenous
antioxidants induced by cisplatin (cis-diamminedichloroplatinum
II, CDDP) in the testiclular tissue of rabbits. 18 healthy male
New Zealand white rabbits were equally divided into three groups
of six rabbits each, control, cisplatin, and cisplatin+curcumin.
The degree of protection produced by cisplatin was evaluated by
determining the level of malondialdehyde (MDA) and glutathione (GSH),
the activity of catalase (CAT), glutathione peroxidase (GSH-Px),
were estimated from testes homogenates. MDA levels were
increased with cisplatin compared to control but in
cisplatin+curcumin group, MDA levels were found to be lower than
cisplatin group (p<0.05). The activity of CAT and GSH-PX
was decreased in cisplatin and cisplatin+curcumin groups
compared to control (p<0.05). In the case of
cisplatin+curcumin CAT and GSH-Px activity were increased
compared to cisplatin group (p<0.05). GSH levels were
decreased with cisplatin but administration cisplatin+curcumin
increased the levels of GSH comared to cisplatin group (p<0.05).
In
the present study, co-administration of curcumin with cisplatin
prevented the damage to testes induced by this drug
and
may be considered as a potentially useful candidate in the
combination chemotherapy with cisplatin.
Key words:
Rabbit, cisplatin, curcumin, testes, oxidative stress. |
