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Full Length
Research Paper |
Comparison of the tissue distributions of flavonoids after
oral administration of pure baicalin, Radix scutellariae
and Scutellariae-Paeoniae couple extracts to rats
Liu
Wei1, Li Feng1, Zhuang Li1
and Zhang Zhen-Qiu1*
1College
of Pharmacy, Liaoning University of Traditional Chinese
Medicine, 77 Shengming 1 Road, DD Port, Dalian, 116600,
China.
*Corresponding author. E-mail:
zhangzhenqiu@sina.com
Abbreviations: HPLC,
High performance liquid chromatography; TCM,
traditional Chinese medicine; RS, Radix
scutellariae; IS, internal standard; HCl,
hydrochloric acid; FDA, food and drug administration;
QC, quality control; RE, relative error; RSD,
relative standard deviation; LLOQ, lower limit of
quantification; GUS, glucuronidase; LC-MS-MS,
liquid chromatography-mass spectrometry.
Accepted 8 June, 2011
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Abstract |
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Baicalin, baicalein, wogonoside and wogonin are the major
flavones of Radix scutellariae with various beneficial
biological activities. The purpose of this study was to
investigate the tissue distribution and compare the differences
of these flavones after orally administrating the pure baicalin,
R. scutellariae and Scutellariae-Paeoniae couple
extracts with the same dose of 200 mg/kg baicalin. Tissue
concentrations of baicalin, baicalein, wogonoside and wogonin
were determined by high performance liquid chromatography
(HPLC). Organ samples including the heart, liver, spleen, lung,
kidney, large intestine, small intestine and stomach were
collected at three specific times (15 min, 8 h, 24 h). Unpaired
Student’s t-test was used for statistical comparison. The
results indicated that the baicalin, wogonoside, baicalein and
wogonin all occurred in the investigated tissues after orally
administrating the pure compound to the rats. Considerable
concentrations of baicalein and wogonin could be detected in the
investigated tissues, but were not found in rat serum. The
contents of glycosides were higher than that of aglycones in
small intestine, stomach, lung, heart and kidney; conversely in
liver, large intestine and spleen. Pure baicalin was absorpted
faster than R. scutellariae and Scutellariae-Paeoniae
couple extracts. The concentrations of baicalin, baicalein,
wogonoside and wogonin in the investigated tissues after orally
administrating Scutellariae-Paeoniae couple extract (24
h) were higher (P＜0.05)
than after orally administrating the pure baicalin and R.
scutellariae extract.
Key words:
Baicalin, wogonoside, baicalein, wogoin, distribution,
Scutellariae-Paeoniae. |
