Journal of Medicinal Plants
Research Vol. 5(31), pp.
23 December, 2011
ISSN 1996-0875 ©2011 Academic
Antioxidant activities, a-glucosidase inhibitory
effect in vitro and antihyperglycemic of Trapa
acornis shell in alloxan-induced diabetic rats
Wen-yi Kang1*, Yuan-yuan Li1, Xue-zhu
Gu2, Qi-tai Xu1 and Xuan Huang3
of Chinese Materia Medica, Henan University, Kaifeng, Henan,
475004, P. R. China.
of Chinese Materia Medica, China Academy of Chinese Medical
Science, Beijing, 100700, P. R. China.
University College of Medicine, Jiaxing 314001, P. R. China.
*Corresponding author. E-mail:
Tel: (+86)-378-3880680, Fax: (+86)-378-3880680.
Accepted 6 October, 2011
Activity of Trapa acornis shell (TAS), including
antioxidant and α-glucosidase inhibitory activity, was
assayed in vitro. Extracts of ethyl acetate (EtOAC) (TASEA)
(n-BuOH) (TASBU) were studied on protective effects of
alloxan-induced diabetic rats in vivo. The antioxidant
activity was determined by the method of
2,2-azino-bis(3-ethylbenzo-thiazoline-6-sulfonicacid) (ABTS) and
ferric reducing antioxidant power (FRAP) assay. α-Glucosidase
inhibitory activity was screened by 96-microplate-based method.
The results showed that TASEA had the highest antioxidant
activity (DPPH: IC50=1.88±0.04 μg/ml, ABTS: IC50=1.02±0.04
μg/ml and FRAP=8578.25±59.15 μmol TE/g). TASEA had the highest
α-glucosidase inhibitory activity (IC50
=0.42±0.02 μg/ml). Compared with diabetic control mice,
administration of TASEA (1200 and 400 mg/kg) and TASBU (1000 and
300 mg/kg) significantly decreased the level of fasting blood
glucose (FSG), postprandial blood glucose (PBG) and
malondialdehyde (MDA). Oral administration of TASEA and TASBU
could decrease the level of triglyceride (TG) without
statistical significance. TASEA (1200 mg/kg) could significantly
increase liver glycogen content and decreased the level of total
cholesterol (TC) and TASBU (1000 mg/kg) could significantly
increase the level of superoxide dismutase (SOD) in serum. The
results indicate that TASEA and TASBU had signiﬁcant
antihyperglycemic effects in alloxan-induced diabetic rats.
Trapa acornis shell, antioxidant activity, α-glucosidase
inhibitory activity, antihyperglycemic, diabetes.