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Journal of Medicinal Plants
Research Vol. 5(14), pp.
3329–3334,
18 July, 2011
ISSN 1996-0875 ©2011 Academic
Journals |
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Full length
Research Paper |
Optimization
of Jatropha curcas for total
flavonoids by response surface methodology and
antibacterial activity
Zhang Sheng-tan1#,
Cheng Guo-hua2#, Lin Jing-ming1#, Wang
Tie-shan1, Zheng Ni1 and Wang Zhao-yu3*
1Department
of Pharmacy, Zhujiang Hospital, Southern Medical University,
Guangzhou, 510282, China.
2Department
of Pharmacy, Cancer Hospital, Guangzhou Medical University,
Guangzhou, 510095, China.
3College
of Life Science and Biopharmacology, Guangdong
Pharmaceutical University, Guangzhou, 510006, China.
*Corresponding author. E-mail:
linjm1231@21cn.com.
Tel: 86-20-61643557.
Fax: 86-20-61643010.
Accepted 5 April, 2011
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Abstract |
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Jatropha curcas
is known as a member of the Euphorbiaceae plants. In this study,
using rutin in determining indicators, the extraction technology
of total flavonoids was optimized by the ultrasonic assisted
method with response to surface methodology from Jatropha
leaves, and the antibacterial effects of total flavonoids were
studied. Results of the response surface method showed that
under the extraction conditions of 70.05% ethanol, the
solid-liquid ratio of 1:28.51 and the extraction time of 39.95
min were extracted twice. Consequently, the best extraction
efficiency of the total flavonoids (rutin calculated) could be
obtained and the predictive value was 0.705%, while the actual
average extraction rate was 0.691%.
The results of the
inhibitory effect for bacteria and fungi showed that total
flavonoids had remarkable inhibitory effects on Trichonphyton
mentagrophyton, Staphyloccocus aureus, Enterococcus
and Rosenbach faecalis, and they had
no inhibitory effect on the G-bacteria.
Key words:
Jatropha curcas,
total flavonoids, response surface methodology, antibacterial
activity.
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