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Journal of Medicinal Plants
Research Vol. 5(4), pp.
507–513,
18 February, 2011
ISSN 1996-0875 ©2011 Academic
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Full Length Research Paper |
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Shatavarin
production from in vitro cultures of Asparagus
racemosus Wild
Mashitha Pise, Jayshree Rudra, Sunita Bundale,
Deovrat Begde, Nandita Nashikkar and Avinash Upadhyay*
Hislop School of Biotechnology, Hislop College, Temple Road,
Civil Lines, Nagpur, Maharashtra – 440001, India.
*Corresponding author. E-mail:
pmmashi@gmail.com,
avinash_u@yahoo.com.
Accepted 20 May, 2010 |
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Abstract |
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Asparagus racemosus
is one of the important medicinal plants found in India, China
and other parts of the world. This plant is known to produce
steroidal saponins called Shatavarins. Callus cultures of A.
racemosus were initiated in a modified MS medium
supplemented with 1.0 mg/L NAA and 2, 4-D and 0.5 mg/L BAP and
compared for growth and production of saponin over a period of
60 days. Saponin production was evaluated at a regular interval
of 15 days. Root calli produces more saponin compared to nodal
calli and maximum accumulation was found to be 10.38 ± 0.14 mg/g
of callus after 60 days of inoculation. Total saponins from the
nodal calli were found to be 7.69 ± 0.136 mg/g of callus.
Compared to wild type roots, in vitro cultures showed 20
fold increases in shatavarin levels. High performance liquid
chromatography (HPLC) chromatograms of the cultures indicated
that the overall saponin profile of in vitro and in
vivo plant root extract is similar.
Key words:
Tissue culture, secondary metabolites, multiple growth
regulators, saponins.
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