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  J. Med. Plants Res.

 

  Vol. 5 No. 4
 

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Ameri A

Deokule SS

 

 
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 Journal of Medicinal Plants Research Vol. 5(4), pp. 527–532, 18 February, 2011

 ISSN 1996-0875 ©2011 Academic Journals  

   

 

Full Length Research Paper

 

  
 

Anti-Staphylococcus aureus activity of Pisolithus albus from Pune, India

 

Abdulghani Ameri1, Chanchala Ghadge2, Jitendra G. Vaidya3 and Subhash S. Deokule3

 

1Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahwaz, Iran, 61357-3318

2Department of Statistics, University of Pune, Pune-411007, India.

3Department of Botany, University of Pune, Pune-411007, India.

 

*Corresponding author. E-mail: kazem1920@hotmail.com. Tel: +98 9166113274.

 

Accepted 2 February, 2011

  
     
 

 Abstract

  
     

In vitro studies were conducted to investigate the anti-staphylococcal activity of crude extracts of two strains of Pisolithus albus collected from different hosts in the vicinity of Pune University Campus, Pune, India. Organic solvents of increasing polarities were used for extraction of secondary metabolites. In addition, bioactive components of known antimicrobial activity were isolated and tested against thirty strains of clinical isolates of methicillin resistant Staphylococcus aureus. Strong activity was exhibited by ethyl acetate and methanol extracts while aqueous extract showed weak activity. All strains were resistant to the chloroform extract. Maximum effectiveness was recorded for sesquiterpenoids extract (zone diameter: 16.67 to 31.5 mm). Triterpenoids and diterpenoids extracts displayed weak to moderate activity. Polysaccharides fractions IIIa and IIIb were weakly effective while fractions I and II did not show activity. Minimal inhibitory concentration was determined to be in a range of 0.62 to 1.2 mg/ml.

 

Key words: Pisolithus albus, Staphylococcus aureus, methicillin resistant antimicrobial activity.

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