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Journal of Medicinal Plants
Research Vol. 5(18), pp.
4647-4653,
16 September, 2011
ISSN 1996-0875 ©2011 Academic
Journals |
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Full Length
Research Paper |
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Antioxidant and cytotoxic activity of the acetone extracts of
root of Euphorbia hylonoma and its ellagic acid
derivatives
Zengjun Guo*, Ying Xu, Ling Han, Xiaoqian Bo, Chen Huang and Lei
Ni
Faculty of Pharmacy, Medical School of Xi’an Jiaotong
University, Xi’an 710061, China.
*Corresponding author. E-mail:
guozj@mail.xjtu.edu.cn. Tel: +86-29-82655133.
Fax: +86-29-82655424.
Abbreviations: DPPH ,1,
1-Diphenyl-2-pycrylhydrazyl; MTT,
thiazolyl tetrazolium;
DMSO,
dimethyl sulfoxide;
EDTA,
ethylenediaminetetraacetic
acid;
IC50,
inhibition concentration; FBS, fetal bovine serum;
PBS, phosphate buffer saline.
Accepted 15 July, 2011 |
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Abstract |
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The acetone extracts and fractions prepared from the roots of
Euphorbia hylonoma were investigated for
1, 1-diphenyl-2-pycrylhydrazyl (DPPH) free radical,
superoxide anion and hydroxyl radical scavenging activity and
cytotoxic activity against the human hepatoma cell line
SMMC-7721, human cervix epitheloid carcinoma cell line HeLa and
the human gastric cancer cell line SGC-7901. The acetone extract
and fraction of EtOAc showed high antioxidant activities. The
acetone extract and fraction of water showed a stronger
cytotoxic effect on all tested cells, with IC50 values being
less than 20 μg/mL. The acetone extract and fraction of water
induced cell cycle arrest in G1 phase in SMMC-7721 cells. The
major compounds isolated from the acetone extract were ellagic
acid derivatives and showed weak cytotoxic activities on the
tested cell lines, and these compound were 3,3,4-tri-O-methylellagic
acid (1), 3,3-di-O-methylellagic acid
(2), 3-O-methylellagic acid (3) and 3,3-di-O-methylellagic acid-4-O-β-dxylopyranosid (4).
Compound 3 showed better obvious radical scavenging activity
than other tested compounds.
Key words:
Euphorbia hylonorma
Hand-Mazz, antioxidant activity, cytotoxic activity, chemical
constituents. |
