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  J. Med. Plants Res.

 

  Vol. 5 No. 18
 

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  articles by:
 

  Abdelmageed AHA

  Kadir MA


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 Journal of Medicinal Plants Research Vol. 5(18), pp. 4465–4469, 16 September, 2011

 ISSN 1996-0875 ©2011 Academic Journals  

 

 

Full Length Research Paper

Micropropagation of Etlingera elatior (Zingiberaceae) by using axillary bud explants

 

A. H. A. Abdelmageed1,4, Q. Z. Faridah1,2*, F. M. A. Norhana1, A. A. Julia2 and

Midhzar Abdul Kadir3

 

1Biodiversity Unit, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

2Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

3Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

4Department of Horticulture, University of Khartoum, Shambat 13314, Khartoum North, Sudan.

 

*Corresponding author. E-mail: faridahqz@gmail.com. Tel: +60389471413.

Fax: +6038947141.

 

Accepted 24 February, 2011 
 

 Abstract
 

Axillary bud explants of Etingera elatior were cultured on Murashige and Skoog (MS) basal medium, supplemented with various concentrations of cytokinin (BAP) or auxin (IAA) as plant growth regulators. The addition of 22.2 µM BAP to the MS medium produced the highest mean number of shoots (3.67) per explants as compared to other concentrations. The best shoots length (4.20 cm) was obtained from the medium containing 26.6 µM of BAP. For in vitro rooting of shoot, MS medium supplemented with 11.4 µM IAA produced the highest number of root, whereas 34.2 µM IAA gave the longest roots in the medium. The proliferated shoots were green and healthy in appearance. The Plantlets with well developed roots were hardened, acclimatized and planted out successfully with a survival rate of 75%.

 

Key words: Etlingera elatior, axillary bud, medicinal plant, micro propagation, plant growth regulator, Zingiberaceae.

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