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RP-HPLC analysis for camptothecin content in Nothapodytes
nimmoniana, an endangered medicinal plant
Indramohan Singh*, N. Kumaravadivel, R. Gnanam and S.
Vellaikumar
Department of Plant Molecular Biology and Biotechnology,
Centre for Plant Molecular Biology, Tamil Nadu Agricultural
University, Coimbatore, India.
*Corresponding author. E-mail:
inder191@gmail.com.
Accepted 4 December, 2009 |
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Studies
were carried out on analysis of secondary metabolites by RP-HPLC from
different parts (both in- vitro and -vivo) of
Nothpodytes nimmoniana, an endangered medicinal plant. HPLC analysis
showed the presence of camptothecin, a medicinally important alkaloid
from N. nimmoniana. The plant parts used for the analysis
include leaves, seeds, seed coat and leaf derived callus. Among all the
plant parts used, seeds recorded maximum (0.040%) camptothecin content
followed by dried leaf samples. Seed coat recorded least camptothecin
content. The study indicated that almost all parts of the plant contain
camptothecin.
Key
words:
Nothapodytes nimmoniana, Icacinaceae, HPLC, secondary metabolite,
alkaloid, quinoline, camptothecin. |
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