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Assaying the variation in secondary metabolites of St.
John’s wort for its better use as an antibiotic
Goran S. Nikolic1* and Sasa Z. Zlatkovic2
1Faculty
of Technology, Bulevar oslobodjenja 124, Leskovac 16000,
Serbia.
2Actavis
Trading Ltd, Djordja Stanojevica 12, Novi Beograd 11070,
Serbia.
*Corresponding author.
E-mail:
goranchem_yu@yahoo.com.
Tel/ Fax: +381 16 242 859.
Accepted 29 December, 2009 |
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The
present study is aimed at investigating the effects of variation in
secondary metabolites of St. John’s wort for its better use as an
antibiotic. The
seasonal dynamics
investigation
of St. John’s wort secondary metabolites was carried out on
annual, biennial and triennial wild-growing plants of the suburban
localities, and on the indigenous perennial plants of the mountainous
localities. The effects of variation in secondary metabolites of the
plant material were monitored using a complex antimicrobial preparation
imanin. As plant
secondary metabolites, imanin was isolated from
flowers and leaves of
St.
John’s wort by aqueous-alkaline extraction. The quality of imanin
contained in
St. John’s wort was determined by FTIR and HPLC
methods.
The imanin extracts were tested for antimicrobial activity against
Staphylococcus aureus, Streptococcus agalactiae, Bacillus
diphteriae, Bacillus tetani, Clostridium
histolyticus, Bacillus mesentericus and Bacillus
mycoides.
The quantitative effects of temperature and light intensity
on imanin accumulations in St. John’s wort were examined depending on
the sampling periods and location. The results of antimicrobial activity
and quantitative effects of climatic conditions were correlated with
vegetation phases of hypericum plants.
Key
words:
Imanin,
St. John’s wort, antimicrobial activity,
hyperforin, plant vegetation. |
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