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  J. Med. Plants Res.

 

  Vol. 3 No. 9
 

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  articles by:
 

 Hassan A

 Amjid I

 


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Journal of Medicinal Plants Research Vol. 3 (9), pp. 670–673, September 2009

ISSN 1996-0875 © 2009 Academic Journals  

   

 

Full Length Research Paper

 

 
 

Gas chromatography-mass spectrometric studies of stems essential oil of Pinus roxburghaii and their antibacterial and antifungal activities

 

Ammara Hassan1 and Imran Amjid2

 

1 Pakistan Council of Scientific & Industrial Research Labs. Complex.Lahore. E. mail: ammara.pcsir@gmail.com

2 Haleeb Foods Pvt Ltd, Lahore. E.mail: rao.imran@haleebfoods.com

 

*Corresponding author. E-mail: ammara.pcsir@gmail.com

 

Accepted 29 June, 2009

 
     
 

Abstract

 
     
 

Essential oil of Pinus roxburghaii stems was analyzed by GC MS and their antibacterial activity was studied. Seventeen components out of fifty-two were identified in the oil. Major component in essential oil was α- pinene (41.9%) followed by 3-carene (16.3%), caryophyllene (12.3%), p-cymene (1.9%), Terpinenol (1.8%), Limonene (1.7%), Borneol acetate(1.1%), Caryophyllene oxide (1.0%),camphene (0.9%),Tepinyl acetate (0.8%), β-Phallenderene (0.7%), farnesene (0.6%),o-cymene (0.4%), Butanoic acid, 3-methyl-, 2-phenylethyl ester (0.3%),1-terpinen-4-ol (0.2%), Farnesyle acetate (0.2%) and γ-terpinene (0.2%). Antibacterial activity of stem essential oil was observed against Staphylococcus aureus and Bacillus subtilis while no activity was observed against E. coli and Enterobacter aerogenes. Similarly, antifungal activity of Pinus roxburghaii essential oil was also found to be active against Aspergillus terrus, Aspergillus flavus, Aspergillus candidus, Aspergillus vessicolor, Aspergillus niger and Trichoderma viride.

 

Key words: Pinus roxburgahii, antibacterial activity, antifungal activity, α-pinene, caryophyllene.

 

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