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  J. Med. Plant Res

 

  Vol. 3 No. 5
 

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 Obi RK

 Orji NM

 


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Journal of Medicinal Plants Research Vol. 3 (5), pp. 429432, May, 2009

ISSN 1996-0875 © 2009 Academic Journals  

   

Full Length Research Paper

 

 

 
 

Antibacterial qualities and phytochemical screening of the oils of Curcubita pepo and Brassica nigra

 

R. K. Obi1*, F. C. Nwanebu1, U. U. Ndubuisi2 and N. M. Orji3

 

1Department of Microbiology, Federal University of Technology, Owerri, P. M. B. 1526, Owerri, Imo State, Nigeria.

2Department of Microbiology, University of Uyo, P. M. B. 1017, Uyo, Akwa Ibom State, Nigeria.

3Department of  Biological Sciences, Anambra State University, Uli, P. O. Box 02, Uli, Anambra State, Nigeria.

 

*Corresponding author. E-mail: robertobi_2003@yahoo.ca.

 

Accepted 15 May, 2009

 
     
 

Abstract

 
     
 

The oils of 2 seeds, Brassica nigra (black mustard) and Curcubita pepo (flutted pumpkin) obtained from St. Paul’s catholic church, Owerri and Ihiagwa market respectively, were extracted using the Soxhlet extractor. The antibacterial activities of the extracted oils on 2 fresh hospital isolates, Staphylococcus aureus and Escherischia coli were determined using the Kirby Bauer disc diffusion method of sensitivity testing. Results obtained showed that the oil from C. pepo produced a higher zone of inhibition of 60% on S. aureus which is higher than that of the oil of B. nigra with a zone of inhibition of 40%. However, these 2 oil extracts showed similar inhibitory effects on E. coli. Results of phytochemical analyses of the extracts reveal that the oil from C. pepo contains tannins, flavonoids, saponins, cyanogenic glycosides and cardiac glycosides, while the oil from B. nigra consists of tannins, flavonoids and alkaloids. These seeds, apart from their roles as food additives and supplements, they may also be utilized as effective and cheap sources of antibacterial agents for the treatment of bacterial infections.

 

Key words: Oil, Escherischia coli, Staphylococcus aureus, antibacterial, Curcubita pepo, Brassica nigra.

 

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