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Full Length Research Paper
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Presence of antibiotic
resistant coliforms in sachet water sold in some parts of
South Eastern Nigeria
Afiukwa, Ngozi Felicitas1, Iroha Ifeanyichukwu
Romanus1*, Afiukwa Celestine Azubuike1,
Ayogu Thomas Eze2,3, Onwa Ndubuisi Collins1
and Nwuzo Agabus Chidiebube1
1Department
of Applied Microbiology, Faculty of Biological Sciences,
Ebonyi State University, P. M. B. 053, Abakaliki, Ebonyi
State.
2Department
of Biotechnology, Faculty of Biological Sciences, Ebonyi
State University,
P. M. B. 053, Abakaliki, Ebonyi State.
3Department
of Food Technology, Institute of Management and Technology,
Enugu, P. M. B. 01079, Enugu State, Nigeria.
*Corresponding author. E-mail:
ifynero@yahoo.com.
Accepted
14 August, 2010 |
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Abstract |
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Three
hundred sachet-water samples produced in Enugu and Onitsha
namely: Aqua Rapha, Bejoy, Teck Tack, SO and Rock Tama were
collected from six different batches. They were screened for
the presence of faecal coliforms using standard microbiology
techniques. Sachet- water samples contaminated with faecal
coliforms were selected and tested for their susceptibility
to fourteen different antibiotics by disc diffusion method.
80 (26.6%) were contaminated with faecal coliforms as
follows: Aqua Rapha 25 (41.6%) specifically (Eschericha
coli, Enterobacter and Klebsiella spp.),
Rock Tama 1 (1.6%) (E. coli,), Bejoy 20 (33.3%) (E.
coli, Enterobacter and Klebsiella spp.) ,
Teck Tack 9 (15%) (E. coli, Enterobacter and
Klebsiella spp.), and SO 33 (55%) (E. coli,
Enterobacter and Klebsiella spp). Susceptibility
studies showed that E. coli was highly resistance to
trimethroprim, sulphamethoxazole/trimethroprim, ampicillin,
aztrenoman, ceftazidime, and cefpirome but susceptible to
gentamicin, ciprofloxacin, cefoxitin and cefotaxime.
Enterobacter and Klebsiella spp. were totally
resistance to all the antibiotics tested. We report the
presence of multi-drug resistance fecal coliform in the form
of E. coli, Enterobacter and Klebsiella
spp. from sachet- water.
Key
words:
Sachet water, feacal coliform, antibiotics, multi-drug
resistance. |
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