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Ultra-low doses of melafen affect the energy of mitochondria
I. V. Zhigacheva1, E. B. Burlakova1,
I. P. Generozova2, A. G. Shugaev2 and
S. G. Fattahov3
1Russian Academy of Sciences, N. M. Emanuel Institute
of Biochemical Physics, ul. Kosygina 4, 119334 Moscow,
Russia.
2Russian Academy of Sciences, K. A. Timiryazev
Institute of Plant Physiology ul. Botanicheskaya 35, 127276,
Moscow, Russia.
3Russian Academy of Sciences, A. E. Arbuzov Institute
of Organic and Physical Chemistry, ul. Akademika Arbuzova 8,
420083 Kazan’ Research Center, Kazan’, Tatarstan, Russia
*Corresponding author. E. mail: zhigacheva@mail.ru. Tel:
+7495-939-74-09. Fax: +74991374101.
Accepted
16 November, 2009 |
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Addition
of the organophosphorous plant growth regulator - melafen to
the mitochondria incubation medium resulted in modification
of energy thereof. The modification was dose dependent one.
The melafen concentrations 2
×
10-12, 2
×
10-18 and 2
×
10-21
М
raised
the maximum rates for oxidation of NAD+-dependent
substrates, elevated the efficiency of oxidative
phosphorylation and activated electron transport in the
terminal step of mitochondrial respiratory chain. Melafen
stimulated electron transport during oxidation of succinate
by rat liver mitochondria, but had no effect on the rate of
this substrate oxidation by sugar beet root mitochondria,
which was an evidence for adaptive properties of the
preparation. Water stress resulted in decreasing the maximum
rates of oxidation of NAD+-dependent substrate
and decreasing the electron transport rates at the end of
the respiratory chain by 30%. A pretreatment of pea seeds
with a 10-7% solution of melafen led to
elimination of differences in energetic of mitochondria of
sprouts growing under standard conditions and under
conditions of low moisture. By stimulating the activity of
NAD+-dependent dehydrogenases and activation
electron transport in the cytochrome oxidase part of
respiratory chain, melafen stimulated energy metabolism in
the cells and these effects determine the adaptive
properties thereof.
Key
words:
Cytochrome oxidase, mitochondria, oxidative phosphorylation,
respiratory chain. |
