Sequencing and identification of homologous region encoding rust resistant-gene in soybean (Glycine max L.)

Nguyen Thi Lang¹*, Nguyen Cao Quan Binh¹ and Bui Chi Buu²

¹Genetic and Plant Breeding Department, CuuLong Delta Rice Research Institute, lai, CanTho City, Vietnam.

²Plant Breeding of Southern Agricultural Science Institute (IAS), 121, Nguyen Binh Khiem, TP Ho Chi Minh, Asia.

*Corresponding author. E-mail: ntlang@hcm.vnn.vn.

Accepted 29 October, 2009

   Abstract

Soybean (Glycine max L.) is one of the most important crops that is both easily cultivated and of high efficiency. Its product is commonly used as fresh-seed directly or produced into plant oil, cake, candy and milk. It can also be processed as feed for domestic animals for enhancing the protein requirement in daily meals, and it can be exported for providing valuable currency resource. On the other hand, it is also used in the process of land improvement, contributing to increase yield. Soybean is not only useful in medicine for treatment of human disease, but it is also utilized to provide materials for the processing of animal feed with rich-protein resource and also for industrial application. Based on the result of phenotypic evaluation in rust disease, 81 varieties of soybean were classified into three groups: group A consists of 37 varieties with resistance level of 0 and 1; group B has 30 varieties with resistance level of 3 and 5; and group C has 14 varieties with resistance level of level 7 and 9. We have designed two primers for targeting coding region of rust resistant-gene, represented by Langrisat1 and Langrisat2. We have shown that the soybean varieties in group A have high rust resistance capability, which can provide further application in breeding programming. A key task for the future is to determine whether rust resistant-gene can be transferred to susceptible soybean varieties. Two designed primers are needed for synthesis and testing through Polymerase Chain Reaction (PCR) upon request. To the best of our knowledge, the results presented here are the first characterization of nucleotide of rust resistant-gene in soybean. Further analysis is necessary for identifying nucleotide sequence and its functional site accurately.

Key words: Rust resistant gene, soybean, Glycine max L., sequencing.