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Full Length Research Paper
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Asymptomatic bacteriuria in patients with type-2 diabetes
mellitus
E. A. Ophori1*, P. Imade2 and E. J.
Johnny3
1Department of Microbiology,
University of Benin, Benin City, Edo State, Nigeria
2Department of Medical Microbiology,
University of Benin Teaching Hospital, Benin City, Edo
State, Nigeria.
3University of Benin Teaching
Hospital, Benin City, Edo State, Nigeria.
*Corresponding author. E-mail:
eaophori@yahoo.com.
Accepted 6 April, 2010 |
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Abstract |
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This study was to investigate the
prevalence of asymptomatic bacteriuria (ASB) in patients
clinically diagnosed with type 2 diabetes mellitus and to
determine the antibiotic sensitivity pattern of bacterial
isolates. One hundred and thirty type 2 diabetics comprising 56
males and 74 females (aged between 30 - 59 years) attending the
Central Hospital, Benin- City, Nigeria were studied. Mid-stream
urines were collected from patients who gave informed consent
aseptically into sterile McCartney bottles and examined
microscopically, culturally using standard techniques and tested
for glucose, post-prandial glucose, protein and ketone using a
dipstick. Samples were cultured on blood agar, McConkey agar and
cysteine lactose electrolyte deficient (CLED) media and
incubated at 37°C aerobically for 24 h. Isolates were tested
against antibiotics which included tetracycline,
chloramphenicol, ciprofloxacin and cotrimoxazole by the disc
diffusion method. White blood cells (WBC) and red blood cells (RBC)
were detected in 87 and 6% of samples while ketones and proteins
were 6% and 96% respectively present in the samples. Significant
bacteriuria (≥105 cfu /ml) was observed in some
samples. Bacteria isolated included Escherichia coli with
a prevalence of 56.9%, followed by Klebsiella pneumoniae
(12.7%), Staphylococcus aureus (8.5%) and Proteus
sp. (6.3%). E. coli, K. pneumoniae, S. aureus and
Proteus sp. were most sensitive to cotrimazaxole,
amoxicillin, nalidixic acid and ciprofloxacin but a large number
of bacteria were resistant to tetracycline, chloramphenicol and
ampicillin. The misuse of some antibiotics is a major factor
responsible for bacterial resistance. Therefore, treatment of
ASB in diabetics must be by drugs prescribed by physicians after
proper laboratory analysis.
Key words: Bacteriuria, type 2 diabetes mellitus, antibiotics,
Nigeria, white blood cell, red blood cell.
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