|
Full Length Research Paper
|
|
Optimization of Helicobacter
pylori culture in order to prepare favorable antigens
Esmaeilli Davood1, Mohabati Mobarez Ashraf1*,
Salmanian Ali Hatef2 and Zavaran Hosseini Ahmad3
1Tarbiat
Modares University, Faculty of Medical Science, Department
of Bacteriology Tehran, Iran.
2National Institute for Genetic Engineering and
Biotechnology, Iran.
3Tarbiat Modares University, Faculty of Medical Science,
Department of Immunology Tehran, Iran.
Accepted 11 November, 2009 |
|
|
|
Abstract
|
|
|
Helicobacter
pylori is gram-negative and microaerophilic bacterium. This
bacterium is prevalent worldwide and more than half of the world
populations are infected with H. pylori. The growth of H. pylori
in proper media and nutritional requirements have only partially
been defined. H. pylori growth in proper media and conditions
and preparation of desired antigens without changes in their
structure is essential. H. pylori are generally grown on complex
media supplemented with blood, serum, proper antibiotic. In
broth culture, it exists in the spiral form and transforms into
the coccoid form under certain conditions. Here we modified
transport, growth and preservation media in which all the
constituents are defined, allowing each of them to be
selectively omitted or further components added.
Key words: Optimization, Helicobacter pylori, culture.
|
