two-photon excitation methods for determination of Ca2+
in contamination with protein
A. S. M. Noor1*, A.
Miyakawa2, W. Inami3 and Y. Kawata2
and Photonics Network Centre of Excellent (WiPNet),
Department of Computer and Communication System Engineering,
Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM
of Mechanical Engineering, Faculty of Engineering, Shizuoka
University, Johoku 3-5-1, Naka-ku, Hamamatsu, Shizuoka
of Global Research Leaders, Shizuoka University, Johoku
3-5-1, Naka-ku, Hamamatsu,
Shizuoka 432-8561, Japan.
*Corresponding author. E-mail:
Accepted 8 September, 2011
Three emission wavelengths from two-photon excitation
techniques were use
using a new analytical calculation. This was achieved by
interaction of protein with
Ca2+ and indo-1 in protein contaminated sample.
The emissions obtained from the excitation with the
dissociation constants were used in the calculation.
Agreement values of the Ca2+ from the calculation
with the known Ca2+ buffer solution used
the measurement was obtained for various Ca2+ and
protein concentration. The analytical analysis shows that
deviation errors are small to affect free Ca2+
determination. This would provide an accurate measurement of
free Ca2+ in biological cells than the typical
two emission wavelength processes. Furthermore, using
two-photon excitation methods, deep inside cells observation
of free Ca2+ are possible using the methods
Free Ca2+, indo-1, two-photon excitation methods.